Niagara University is located on Route 104 on the northern limits of the city of Niagara Falls. The 160-acre main campus runs along the top of picturesque Monteagle Ridge overlooking the Niagara River Gorge, just four miles north of the world-famous waterfalls. The university's campus is adjacent to the international border between the United States and Canada and within a short drive of Buffalo and Toronto, creating an international milieu.

In addition to numerous scenic attractions associated with and just outside of Niagara Falls, many other important educational and cultural resources in the area include the Earl W. Brydges Artpark, Old Fort Niagara, The Buffalo Theater District, the Buffalo Philharmonic Orchestra, the Niagara Falls Little Theatre and the Albright-Knox Art Gallery. Niagara University is easily accessible via the New York State Thruway, and is centrally located for bus and airline transportation.